Our science
RNA research and applications have been underpinned by in vitro transcription (IVT). However, RNA impurity resulted from the byproducts of the enzymatic synthesis can severely impede downstream applications.
At RiboTech, we strive to provide innovative enzymes that enable synthesis of RNAs with minimal byproducts. Our discovery engine is powered by: (1) systematic characterization of RNA polymerases from the rich diversity of natural existence; (2) knowledge-driven engineering of existing RNA polymerases towards improved biochemical properties.
Featured products include: (1) CleanT7™ RNA polymerase, which is an engineered mutant of the T7 RNA polymerase (Bingbing Yu et al., 2024). CleanT7™ RNAP significantly reduces full-length dsRNA during routine RNA synthesis. (2) IceLake™ (VSW-3) RNA polymerase is a novel RNA polymerase encoded by a psychrophilic phage VSW-3 from the Napahai located in Shangri-La County in the southwest of China (Heng Xia et al., 2022). IceLake™ RNAP is capable of carrying out in vitro RNA synthesis at low temperature (4-25℃) with minimal dsRNA byproduct (Guoquan Wang et al., 2022).
Our motto is "Clean RNA synthesis by Nature". We hope that our products can be useful to your research.
References:
- Bingbing Yu, Yifan Chen, Yan Yan, Xueling Lu and Bin Zhu DNA-t erminus-dependent tr anscription b y T7 RNA polymerase and its C-helix mutants. Nucleic Acids Research, 2024 [link]
- , , Yixin Jiang, Rui Cheng, , ,
- Guoquan Wang, Rui Cheng, Qiubing Chen, Yuandong Xu, Bingbing Yu, Bin Zhu, Hao Yin, Heng Xia mRNA produced by VSW-3 RNAP has high-level translation efficiency with low inflammatory stimulation. Cell Insight, Volume 1, Issue 5, October 2022 [link]